Sep. 27 8:30-10:30
Neurochemistry I: amino acid and peptide synthesis and degradation
Dr. Tim Murphy
Fundamental Neuroscience , 1st Ed., Chapter 8, p. 193-234 Chapter 14, p.389-392,
or 2nd Ed. Chapter 7 p. 167-196 and Chapter 13 339-360.
In 3rd Edition: Chap. 7 starting pg.133 and Chapter 12 starting pg. 271.
Cooper, Bloom & Roth , The Biochemical Basis of Neuropharmacology,
Chaps. 7-13, 6th Ed or Chaps 6-11 7th Ed.
Molecular Biology of the Cell , 4th ed. Chapter 11 or Molecular Biology
of the Cell 3rd ed. Chapter 11 p 507-523.
The readings are for
Neurochemistry II also.
Metabolic processes neurons share with other cells and organs.
Enzyme and receptor binding kinetics basics,
competitive and non-competitive inhibition.
Metabolic contingencies imposed by the existence
of a blood-brain barrier (i.e. glucose utilization).
Properties and functions of enzymes and pumps.
Synthesis and metabolism of amino acid neurotransmitters
Neuropeptide synthesis and the pathway to regulated release.
Regulation of catecholamine, indoleamine,
and acetylcholine metabolism.
Synthesis, uptake, release, etc.
Structure specialized for function at two classic synapses
typical CNS synapse
AChR as a model ligand-gated ion channel
single channel and macroscopic currents
subunit composition and structure
Families of ligand-gated ion channels
structure and modulators
inhibitory function in adult
diversity of subunits
scaffolding proteins, signaling enzymes, cell adhesion proteins
AMPA, KA, NMDA classes
Properties of NMDA receptors
++-permeability voltage-dependent Mg
++ block deactivation
Glutamatergic postsynaptic density
approaches to identify components
PDZ domain scaffolding proteins
Synaptic plasticity: hippocampal LTP
R as molecular coincidence detector silent synapses and AMPA
R insertion Synaptic plasticity: other forms
Oct. 7 8:30-10:30
Synaptic transmission I
Dr. Tim Murphy
Fundamental Neuroscience , 1st, 2nd, or 3rd Edition, Chapters 7 and 8 (for Neurochemistry lecture also).
Molecular Biology of the Cell , 4th Ed. Chapter 13.
H. L. Atwood, S. Karunanithi :
Diversification of synaptic strength: presynaptic elements.
Nature Reviews Neuroscience 3, 497 -516 (2002). Advanced review; comprehensive.
T. Galli, V. Haucke, N. R. Gough : Synaptic vesicle fusion
followed by clathrin-mediated endocytosis.
Sci. STKE 2003, tr3 (2003). Shockwave animation
of synaptic vesicle fusion.
T. C. Südhof : The synaptic vesicle cycle.
Annual Review of Neuroscience 2004 Vol. 27: 509-547.
Discovery of chemical transmission.
Criteria for transmitter status.
Ionic requirements for transmitter release: calcium.
Properties of presynaptic calcium channels.
Quantal aspects of release.
Release and recycling of vesicles
Biochemistry of release.
Synaptic vesicle protein cycle
v- and t-SNAREs, common aspects of all secretion
Presynaptic modulation of release.
Activity dependent modulation, autoreceptors, neuromodulation,
readily releasable pool.
Synapses as computational devices.
Presynaptic inhibition, facilitation,
paired pulse depression,
quantal aspects of release etc.
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