Targeting Single Brain Arterioles for Photothrombotic Stroke

Sigler A, Goroshkov A, and Murphy TH (2008) Hardware and methodology for targeting single brain arterioles for photothrombotic stroke on an upright microscope. .
 

Supplemental Movie:

Rose Bengal induced Photothrombosis

Targeting individual brain arterioles for photoactivation of Rose Bengal.  The movie has been recorded as epifluorescence with a CDD camera on a microscope equipped with a 40×, 0.8 NA water immersion lens over a time period of 120 s and shows the blood vessel in sextuple time (movie plays in 20 s) during targeting with 532 nm laser light.  While the laser beam position is fixed (bright spot in the middle of the image), the specimen is being moved to target multiple sites within the blood vessel.  Approximately 80 s after start of photoactivation (~14 s in the movie), the blood flow appears to be blocked.  Diameter of the targeted arteriole: ~40 µm.  Find further description for single frames of this movie in 1 (Fig. 4).  Note that a small amount of background light was been added to permit viewing of areas not subjected to photoactivation; this procedure was only used for creation of this movie and was never employed during an actual experiment. 

1 Sigler A, Goroshkov A, and Murphy TH (2008) Hardware and methodology for targeting single brain arterioles for photothrombotic stroke on an upright microscope. J Neurosci Methods 170:35-44.  Download PDF file: rightclick here.

The movie is availabe to download
in QuickTime format (mov) with the (external link).